FIG 1.

Spatial localization of MmuPV1 E6/E7 expression and E4 and L1 proteins in early lesion formation of infected tissues. (A to D) Time course analysis of early lesion formation of sites infected with MmuPV1 cell-free virus. Each set of panels shows hematoxylin and eosin (HE) staining (top), E6/E7 RNAScope signal (middle), and immunofluorescent detection of E4 and L1 proteins (bottom) at a discrete stage of lesion formation. Staining was carried out on adjacent sections. The nuclei were counterstained with DAPI. The scale is shown with a white bar (100 μm). The boxed areas are enlarged at the bottom (A and B). The dotted lines indicate the position of the basal layer.