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80S ribosome assembly on hinge mutant IAPV IGR IRESs. Wild type and the indicated mutant [32P]-IAPV IGR IRESs (0.5 nM) were incubated with increasing amounts of purified salt-washed 80S ribosomes, and the fractions bound were quantified by filter binding assay and phosphorimager analysis. The TM IAPV IRES denotes mutations that disrupt all three pseudoknots (25). Shown are averages ± standard deviation from at least three independent experiments.
