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. 2022 Mar 9;96(5):e01813-21. doi: 10.1128/jvi.01813-21

FIG 6.

FIG 6

The recruitment of membrane-bending proteins to the CCS required SM-cholesterol complex domains. (A to G) Typical confocal images of the basal PM used for testing the colocalization of membrane-bending proteins (Epsin1, FCHo2, FBP17, CIP4, pacsin2, EndoA2, and SNX9) with the CCS before (intact) and after (Depl) SM-cholesterol complex depletion in live cells with MβCD for 30 min. Epsin1, 9 cells (intact and depletion); FCHo2, 10 cells (intact) and 11 cells (depletion); FBP17, 17 cells (intact) and 11 cells (depletion); CIP4, 16 cells (intact) and 17 cells (depletion); EndoA2, 11 cells (intact) and 8 cells (depletion); pacsin2, 10 cells (intact) and 7 cells (depletion); SNX9, 18 cells (intact) and 14 cells (depletion). (H and I) Number densities of fluorescent spots of membrane-bending proteins (H) and fractions of the CCS colocalized by the fluorescent spots of membrane-bending proteins (I) on the basal PM and the dorsal PM at a height of ∼1 μm from the basal PM before and after cholesterol depletion.