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. 2022 Mar 9;96(5):e02120-21. doi: 10.1128/jvi.02120-21

FIG 2.

FIG 2

Evaluation of the binding specificities of single mutants of the HA of A/Turkey/Italy/214845/02 H7N3. (A) Synthetic glycans printed on the microarray (n = 6), either without sialic acid (structures 1 to 3; light gray), with α2,6-linked NeuAc (structures 4 to 6; black), α2,3-linked NeuAc (structures 7 to 9; dark gray), α2,6-linked NeuGc (structures 10 to 12; red), or α2,3-linked NeuGc (structures 13 to 15; blue). Structures 1, 4, 7, 10, and 13 contain one LacNAc repeat, structures 2, 5, 8, 11, and 14 have two repeats, and structures 3, 6, 9, 12, and 15 contain three repeats (17). The glycan microarray, which is representative of two independent assays, was used to determine the receptor specificity of recombinantly expressed HA of the H7tu wild type (WT) (B) and S128T (C), I130V (D), A135E (E), G144R (F), A159G+A160V (G), T189A (H), K193R (I), and A219P (J) mutants.