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. 2022 Mar 7;219(4):e20211838. doi: 10.1084/jem.20211838

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© 2022 Fang et al.

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Figure S1. — Analysis of HBV-specific CD8⁺ cells in PBMCs from CHB patients. (A) Detecting HLA-A2 expression on PBMCs from CHB patients. (B) Gating strategy of flow cytometry for HBsAg-specific (Env335-343) and HBcAg-specific (Core18-27) CD8⁺ T cells from PBMCs. (C) Analysis of HPV-specific CD8⁺ cells from three HLA-A2–positive CHB patients. (D and E) Comparing the frequencies of HBV polymerase–specific (D) or CMV pp65–specific (E) CD8⁺ T cells between subjects at different ages. (F) Correlating age with the frequency of mMDSCs in in CHB patients (n = 40). (G and H) Correlated age with the frequency of mMDSCs in in CHB patients at age > 18 (n = 20; G) or at age ≤ 18 (n = 20; H). *, P < 0.05; ***, P < 0.001.