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. 2022 Mar 7;219(4):e20211838. doi: 10.1084/jem.20211838

Figure S3.

Figure S3.

Migration of mMDSCs. (A) Transwell migration study of mMDSCs from CHB patients toward hCCL19 or hCXCL12 (n = 3, 500 ng/ml). (B) Dose-dependent migration of purified mouse mMDSCs toward agarose dots containing mCCL25, triplicate. Scale bars, 50 μm. (C) Identifying surface markers (CD3, CD19, and Gr-1) on migrated cells from the Transwell study. (D) Purity of mMDSCs using for adoptive transfer (ADT). (E) Detection of allelic cells in the blood from surgically joined CD45.2+ and CD45.1+ mice. (F) Flow cytometry analyzing the expression of CD11c, CD19, Ly6G, and Ly6C on allelic mMDSCs from the parabiosis study. (G) Detection of allelic mMDSCs in the thymus of CD45.1+ mice transferred with CD45.2+ mMDSCs from HBV-persistent HDI mice, IF, triplicate.