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. 2022 Mar 7;219(4):e20211838. doi: 10.1084/jem.20211838

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© 2022 Fang et al.

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Figure S4. — Antigen transfer and thymocyte deletion by mMDSCs. (A) Detection of HBeAg or HBsAg in their stimulated mMDSCs (μg/ml). (B) Detection of the Env183/A2 complex on IL-6 or HBsAg-induced HLA-A2–negative mMDSCs with TCR-like antibody (n = 7). (C) Flow cytometry determining MFI of the Env183/A2 complex on HLA-A2–positive mMDSCs from CHB and CD14⁺ myeloid cells from healthy donors. (D) Flow cytometry determining MFI of the Core18/A2 complex on HLA-A2–positive mMDSCs from CHB and CD14⁺ myeloid cells from healthy donors. (E) IHC staining of HBsAg in the thymus from HBV-persistent HDI mice. Nucleus (violet), Gr-1 (brown), HBsAg (red), IHC, 200X, triplicate. Arrows indicate the positive signaling in the IHC staining. (F) Detection of HBsAg in the thymus from WT and CCR9^−/− HBV-persistent HDI mice (brown, 100X), triplicate. Arrows indicate the positive signaling in the IHC staining. (G) Detecting OVA-specific CD8⁺ thymocytes via flow cytometry in reconstituted mice on day 3 after transfer of OVApp 257-264-loaded mMDSCs (10⁷ cells per mice, n = 5). **, P < 0.01.