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Time-lapse confocal imaging shows the dynamic activation of Rho flares (mCherry-2xrGBD, magenta) at the site of ZO-1 loss (BFP-ZO-1, cyan) and calcium flashes (GCaMP6m, green). Time interval, 5 s; video frame rate, 20 fps. Related to Fig. 2, C and D.
