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Time-lapse confocal imaging of gastrula-stage Xenopus embryo showing whole-field optogenetic stimulation leading to prGEF-mCherry (2xPDZ-mCherry-LARG(DH), gray) recruitment and activation of Rho (mCherry-2xrGBD, gray). First video illustrates whole-field recruitment of prGEF upon light stimulation, and second video illustrates whole-field activation of active Rho upon light stimulation. Time interval, 9 s; video frame rate, 20 fps. Related to Fig. S2, A–D.
