Video 7.
Time-lapse confocal imaging of gastrula-stage Xenopus embryo showing site-specific 405-nm laser light stimulation of junctions expressing optogenetic constructs along with active Rho probe (mCherry-2xrGBD, FIRE LUT) or calcium probe (Halo-C2, FIRE LUT). First video shows increase of active Rho after site-specific light stimulation. Second video shows that local Rho activation is not sufficient to elicit a local calcium increase. Third video shows that upon laser injury of the same junction, there is an increase in local calcium at the site of the wound. White box indicates the region of stimulation. Time interval, 9 s; video frame rate, 10 fps. Related for Fig. 3, C and D.