TABLE 2.
Physicochemical properties of the CARB-6 β-lactamase of V. cholerae and comparison with TEM-1 and other carbenicillin-hydrolyzing enzymes
| β-Lactamase | Mol wt | pIa |
|---|---|---|
| CARB-6 | 33,000b | 5.35 |
| SAR-1 | 33,700b | 4.9 |
| TEM-1 | 28,911c | 5.4 |
| CARB-1 | 31,405c | 5.3 |
| CARB-2 | 31,347c | 5.7 |
| CARB-3 | 31,313c | 5.75 |
a
pI was determined in a polyacrylamide gel with an Ampholine gradient (pH 3.5 to 9.5; Pharmacia).
b
Molecular weight determined by electrophoresis in an SDS–12% polyacrylamide gel by the method of Laemmli (19) with low-range prestained SDS-polyacrylamide gel electrophoresis standards.
c
Molecular weight as estimated according to the amino acid sequence. The molecular mass of CARB-6 calculated according to the amino acid composition is 31,429 Da (Bio-Oriented Web Server Facilities, ABIM-Aix Marseille University).