TABLE 5.
Amino acid point mutations in β-lactamases CARB-1, CARB-2, CARB-3, and CARB-6
| Amino acid positiona | Amino acid changeb
|
|||
|---|---|---|---|---|
| CARB-6 | CARB-1 | CARB-2 | CARB-3 | |
| 192 | Gln | Lys | Lys | Lys |
| 193 | Lys | Phe | Phe | Lys |
| 198 | Thr | Ala | Ala | Ala |
| 202 | Ala | Met | Met | Met |
| 203 | Ser | Asn | Asn | Asn |
| 227 | Val | Ala | Ala | Ala |
| 228 | Lys | Gly | Gly | Gly |
| 230 | Ser | Asn | Asn | Asn |
| 249 | Ile | Val | Val | Val |
| 255 | Glu | His | His | His |
| 256 | Lys | Gln | Gln | Gln |
| 257 | Lys | Ala | Ala | Ala |
| 258 | Thr | Pro | Pro | Pro |
| 269 | Glu | Gln | Gln | Gln |
| 273 | Ala | Glu | Ala | Ala |
| 284 | Arg | His | His | His |
| 288 | Glu | Asp | Asp | Asp |
a
The numbering of the positions of the mutations is according to Ambler (2).
b
Boldface italics indicate mutated amino acids relative to CARB-3 β-lactamase.