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. 2022 Mar 9;8(10):eabl6121. doi: 10.1126/sciadv.abl6121

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Fig. 4. — (A) Screening for neuropeptide receptor genes using IPC-specific RNAi knockdown. Courtship index (top) and single-wing extension number (bottom) was counted between two males of the same genotype. N = 4 to 8 in each group. (B and C) Knockdown of CCAP-R in IPCs induced intermale courtship. Courtship index and single-wing extension number were counted between males. U-CCAP-R-RNAi #2 is another independent RNAi line from the one used in the screening (B). N = 9 to 10 in each group. (D and E) Silencing CCAP cells induced intermale courtship. Courtship index and single-wing extension number were counted between males. N = 8 to 10 in each group. In all analyses, one-way ANOVA followed by Dunnett’s test for multiple comparisons was used, and statistical differences were represented as follows: ***P < 0.001 and ****P < 0.0001. Data were represented as means ± SEM.