Fig. 6. MBH FKBP51 affects sympathetic outflow and peripheral autophagy signaling.
FKBP51 overexpression is depicted in blue, and FKBP51 deletion is depicted in green. (A and B) Representative decrease in tissue NE content after α-MPT injection (left) and turnover rate (right) were determined on SM and eWAT (see fig. S8 for pancreas, heart, iWAT, and BAT tissues). Quantification of (C) pAMPK (T172) and (D) pp70S6K (T389), and (E) p62 level in the SM and eWAT. (F) Representative blots. (G to H) FKBP51 overexpression increased autophagic flux and in SM and eWAT. (I) Representative blots of chloroquine the experiment. Quantification of (J) pAMPK (T172), (K) pp70S6K (T389), (L) LC3B-II, and (M) p62 levels in SM and eWAT in animals lacking FKBP51 in the MBH. (N) Representative blots of FKBP51MBH-KO protein analysis. All data are shown as ±SEM. Protein data are shown as the relative protein expression compared to control. A two-way ANOVA was performed, followed by a Tukey’s multiple comparison test in (F) and (G). For (A) to (E) and (I) to (L), an unpaired Student’s t test was performed. *P < 0.05, **P < 0.01, and ***P < 0.001.