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. 2022 Feb 23;12:788886. doi: 10.3389/fphar.2021.788886

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Copyright © 2022 Veron, Aggarwal, Li, Moeckel, Kashgarian and Tufro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histology of eNOS ^−/−:VEGF^KD kidneys reveals diffuse glomerulosclerosis and mimics advanced DKD: PAS stain representative images: (A) eNOS^−/−:VEGF^KD − dox glomeruli are normal by light microscopy; (B) eNOS^−/−:VEGF^KD + dox glomeruli show microaneurisms (1-2, black arrowheads), mesangiolysis (3,5,6, white arrowheads), mesangial expansion (Papapetropoulos et al., 1997; Shen et al., 1999; Reidy et al., 2014; Tuttle et al., 2014), severe mesangial sclerosis (4-6, black asterisks), proteinaceous tubular casts (4-6, white asterisks) and lymphocytic infiltrates (5–6, yellow arrowheads); Scale bars = 50 μm (A, B1-3) and 100 μm (B4-6); PAS: Periodic acid-Schiff stain.