. 2022 Feb 23;12:788886. doi: 10.3389/fphar.2021.788886

TABLE 1.

General parameters.

	eNOS^−/−:VEGF^KD	eNOS^−/−:VEGF^KD	DM-VEGF^KD	DM-VEGF^KD	VEGF^KD	VEGF^KD
	− dox	+ Dox	− dox	+ Dox	− dox	+ Dox
N	7–9	5	6–9	6–12	4–8	5
Age (days)	130 ± 10*	129 ± 10	198 ± 21*	190 ± 10	135 ± 4	172 ± 1****
BW(g)	25 ± 0.7	24 ± 1.3	31.5 ± 1.3	30.1 ± 1.9	31.5 ± 1***	43 ± 1.2****
KW(mg)	156 ± 12.3**	188 ± 16.8*	254 ± 12****	259 ± 18.8*	223 ± 12	257 ± 14.3
KW:BW ratio (mg/g)	6.3 ± 0.46*	8 ± 0.6*	8.2 ± 0.61	8.3 ± 0.46**	7.1 ± 0.25	6 ± 0.44
Urine volume (ml/day)	0.32 ± 0.02	0.7 ± 0.4	2.5 ± 0.2***	4.1 ± 1.7**	0.27 ± 0.03	0.2 ± 0.04
glycemia (mg/dl)	181 ± 10	165 ± 19	555 ± 29****	458 ± 42****	199 ± 14	198 ± 9
Pl plasma creatinine (mg/dl)	0.09 ± 0.003	0.17 ± 0.033*	0.11 ± 0.022	0.06 ± 0.008**	0.09 ± 0.011	0.09 ± 0.002*

Age: (Welch’s t test): *p = 0.0148, eNOS^−/−:VEGF^KD − dox vs. DM-VEGF^KD − dox; *p = 0.0118, DM-VEGF^KD − dox vs. VEGF^KD − dox; ****p < 0.0001, VEGF^KD − dox vs. + dox. BW: body weight (Welch’s t test): ****p < 0.0001, VEGF^KD − dox vs. + dox; ****p < 0.0001, VEGF^KD + dox vs. DM-VEGF^KD + dox; ***p = 0.0001, VEGF^KD − dox vs. eNOS^−/−:VEGF^KD − dox; ****p < 0.0001 , VEGF^KD + dox vs. eNOS^−/−:VEGF^KD + dox. KW: kidney weight (Welch’s t test): **p = 0.0019, eNOS^−/−:VEGF^KD − dox vs. VEGF^KD − dox; *p = 0.0151, eNOS^−/−:VEGF^KD + dox vs. VEGF^KD + dox; ****p < 0.0001, DM-VEGF^KD − dox vs. eNOS^−/−:VEGF^KD − dox; *p = 0.0153, DM-VEGF^KD + dox vs. eNOS^−/−:VEGF^KD + dox. KW:BW ratio (Welch’s t test): *p = 0.03, eNOS^−/−:VEGF^KD − dox vs. + dox (Mann-Whitney test); *p = 0.03, eNOS^−/−:VEGF^KD + dox vs. VEGF^KD + dox; *p = 0.0272, eNOS^−/−:VEGF^KD − dox vs. DM-VEGF^KD − dox;**p = 0.003, DM-VEGF^KD + dox vs. VEGF^KD + dox. Urine volume: *p < 0.025 (all groups Brown-Forsythe ANOVA test); ****p < 0.0001, DM-VEGF^KD vs. VEGF^KD − dox; **p = 0.0016, DM-VEGF^KD + dox vs. VEGF^KD + dox. Glycemia: ****P=<0.0001 DM-VEGF^KD vs. all non-diabetic mice (Brown-Forsythe ANOVA test). plasma Creatinine: *p = 0.01, eNOS^−/−:VEGF^KD − dox vs. +dox; **p = 0.0043, DM-VEGF^KD + dox vs. eNOS^−/−: VEGF^KD + dox mice; *p = 0.0159, VEGF^KD + dox vs. eNOS^−/−: VEGF^KD + dox (Mann-Whitney test). All other comparisons within and between experimental groups were non significant.