Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (mouse) | Mouse: Ythdf2fl/fl | Yu et al., 2021b | N/A | |
| Strain, strain background (mouse) | Mouse: Tg(Six3-cre)69Frty/GcoJ | Jackson Laboratory | Cat#: JAX_019755RRID: IMSR_JAX:019755 | |
| Strain, strain background (mouse) | Mouse: B6.Cg-Tg(Thy1-EGFP)OJrs/GfngJ | Jackson Laboratory | Cat#: JAX_007919RRID: IMSR_JAX:007919 | |
| Strain, strain background (mouse) | Mouse: B6.129 × 1-Gt(ROSA)26Sortm1(EYFP)Cos/J | Jackson Laboratory | Cat#: JAX_006148RRID: IMSR_JAX:006148 | |
| Antibody | Anti-GFP (Chicken polyclonal) | Abcam | Cat#: ab13970, RRID: AB_300798 |
IF (1:1000) |
| Antibody | Anti-MAP2 (Chicken polyclonal) | Abcam | Cat#: ab5392, RRID: AB_2138153 |
IF (1:10,000) |
| Antibody | Anti-RBPMS (Guinea pig polyclonal) | PhosphoSolutions | Cat#: 1832-RBPMS, RRID: AB_2492226 |
IF (1:1000) |
| Antibody | Anti-VAChT (Goat polyclonal) | Millipore | Cat#: ABN100, RRID: AB_2630394 | IF (1:1000) |
| Antibody | Anti-β Actin (Mouse monoclonal) | Abcam | Cat#: ab6276, RRID: AB_2223210 |
WB (1:30,000) |
| Antibody | Anti-β Actin (Mouse monoclonal) | ABclonal | Cat#: AC004, RRID: AB_2737399 |
WB (1:30,000) |
| Antibody | Anti-AP2α (Mouse monoclonal) | DSHB | Cat#: 3B5, RRID: AB_2313947 | IF (1:1000) |
| Antibody | Anti-Bassoon (Mouse monoclonal) | Enzo Life Sciences | Cat#: ADI-VAM-PS003, RRID: AB_10618753 | IF (1:2500) |
| Antibody | Anti-Brn3a (Mouse monoclonal) | Millipore | Cat#: MAB1585, RRID: AB_94166 | IF (1:300) |
| Antibody | Anti-Calbindin-D-28K (Mouse monoclonal) | Sigma-Aldrich | Cat#: C9848, RRID: AB_476894 | IF (1:200) |
| Antibody | Anti-PKCα (Mouse monoclonal) | Santa Cruz Biotechnology | Cat#: sc-8393, RRID: AB_628142 | IF (1:500) |
| Antibody | Anti-SMI-32 (Mouse monoclonal) | BioLegend | Cat#: 801701, RRID: AB_2564642 | IF (1:200) |
| Antibody | Anti-Strn (Striatin) (Mouse monoclonal) | BD Biosciences | Cat#: 610838, RRID: AB_398157 | IF (1:500) |
| Antibody | Anti-CART (Rabbit polyclonal) | Phoenix Pharmaceuticals | Cat#: H-003–62, RRID: AB_2313614 | IF (1:2000) |
| Antibody | Anti m6A (Rabbit polyclonal) | Synaptic Systems | Cat# 202003, RRID: AB_2279214 |
IF (1:200) |
| Antibody | Anti-melanopsin (Rabbit polyclonal) | Thermo Fisher Scientific | Cat#: PA1-780, RRID: AB_2267547 | IF (1:1000) |
| Antibody | Anti-PKCα (Rabbit polyclonal) | Cell Signaling | Cat#: CST-2056 | IF (1:1000) |
| Antibody | Anti-PSD-95 (Mouse monoclonal) | Abcam | Cat#: ab2723, RRID: AB_303248 |
IF (1:500) |
| Antibody | Anti-Recoverin (Rabbit polyclonal) | Millipore | Cat#: AB5585, RRID: AB_2253622 |
IF (1:1000) |
| Antibody | Anti-YTHDF2 (Rabbit polyclonal) | Proteintech | Cat#: 24744–1-AP, RRID: AB_2687435 | IF (1:1000) |
| Antibody | Anti-YTHDF1 (Rabbit polyclonal) | Proteintech | Cat#: 17479–1-AP, RRID: AB_2217473 | IF (1:1000) |
| Antibody | Anti-YTHDF3 (Rabbit polyclonal) | Abcam | Cat#: ab103328, RRID: AB_10710895 | IF (1:1000) |
| Antibody | Anti-Ubr4 (Rabbit polyclonal) | Abcam | Cat#: ab86738, RRID: AB_1952666 | IF (1:300) |
| Antibody | Anti-Chx10 (Sheep polyclonal) | Exalpha | Cat#: X1179P | IF (1:1000) |
| Antibody | Anti-GFAP (Chicken polyclonal) | Millipore | Cat#: AB5541, RRID: AB_177521 | IF (1:500) |
| Antibody | Anti-Lhx2 (Goat polyclonal) | Santa Cruz Biotechnology | Cat#: sc-19344, RRID: AB_2135660 | IF (1:200) |
| Antibody | Anti-Lhx2 (Rabbit monoclonal) | Abcam | Cat#: ab184337 | IF (1:500) |
| Antibody | Anti-chicken IgY (Alexa 488 donkey) | Jackson Immunoresearch | Cat#: 703-545-155, RRID: AB_2340375 | IF (1:500) |
| Antibody | Anti-G. pig IgG (Alexa 488 donkey) | Jackson Immunoresearch | Cat#: 706-545-148, RRID: AB_2340472 | IF (1:500) |
| Antibody | Anti-mouse IgG (Alexa 488 donkey) | Thermo Fisher Scientific | Cat#: A-21202, RRID: AB_141607 | IF (1:500) |
| Antibody | Anti-rabbit IgG (Alexa 488 donkey) | Thermo Fisher Scientific | Cat#: A-21206, RRID: AB_141708 | IF (1:500) |
| Antibody | Anti-goat IgG (Alexa 555 donkey) | Thermo Fisher Scientific | Cat#: A-21432, RRID: AB_2535853 | IF (1:1000) |
| Antibody | Anti-mouse IgG (Alexa 555 donkey) | Thermo Fisher Scientific | Cat#: A-31570, RRID: AB_2536180 | IF (1:1000) |
| Antibody | Anti-rabbit IgG (Alexa 555 donkey) | Thermo Fisher Scientific | Cat#: A-31572, RRID: AB_162543 | IF (1:1000) |
| Antibody | Anti-sheep IgG (Alexa 555 donkey) | Thermo Fisher Scientific | Cat#: A-21436, RRID: AB_2535857 | IF (1:1000) |
| Antibody | Anti-chicken IgY (Alexa 555 goat) | Thermo Fisher Scientific | Cat#: A-21437, RRID: AB_2535858 | IF (1:1000) |
| Antibody | Anti-mouse IgG (Alexa 647 donkey) | Thermo Fisher Scientific | Cat#: A-31571, RRID: AB_162542 | IF (1:200) |
| Antibody | Anti-mouse IgG (HRP donkey) | Abcam | Cat#: ab97030, RRID: AB_10680919 | WB (1:2500) |
| Antibody | Anti-rabbit IgG (HRP donkey) | Abcam | Cat#: ab16284, RRID: AB_955387 | WB (1:2500) |
| Antibody | Anti-mouse IgG (HRP VHH) | AlpaLife | Cat#: KTSM1321 | WB (1:5000) |
| Antibody | Anti-rabbit IgG (HRP VHH) | AlpaLife | Cat#: KTSM1322 | WB (1:5000) |
| Recombinant DNA reagent | Plasmid: pLKO.1-TRC | Addgene | Addgene plasmid #10878, RRID: Addgene_10878 | |
| Sequence-based reagent | shRNA targeting sequence of negative control |
This paper | N/A | GCATCAAGGTG AACTTCAAGA |
| Sequence-based reagent | shRNA targeting sequence of mouse Ythdf2 |
Yu et al., 2018 | N/A | GGACGTTCCC AATAGCCAACT |
| Sequence-based reagent | shRNA targeting sequence of mouse Ythdf1 |
This paper | N/A | GGACATTGGT ACTTGGGATAA |
| Sequence-based reagent | shRNA targeting sequence of mouse Ythdf3 |
This paper | N/A | GGATTTGGCAA TGATACTTTG |
| Sequence-based reagent | shRNA targeting sequence of mouse Mettl14#6 | This paper | N/A | GCTGGACCTGG GATGATATTA |
| Sequence-based reagent | shRNA targeting sequence of mouse Mettl14#7 | This paper | N/A | CCCAGCTTGT ACTTTGCTTTA |
| Sequence-based reagent | shRNA targeting sequence of negative control (AAV) | This paper | N/A | TTCTCCGAAC GTGTCACGTAA |
| Sequence-based reagent | shRNA targeting sequence of mouse Kalrn12 | This paper | N/A | TGATGAGCTGA TGGAAGAA |
| Sequence-based reagent | shRNA targeting sequence of mouse Ubr4 | This paper | N/A | AATGATGAGC AGTCATCTC |
| Sequence-based reagent | siRNA targeting sequence of negative control | Yu et al., 2018 | N/A | UUCUCCGAAC GUGUCACGUTT |
| Sequence-based reagent | siRNA targeting sequence of mouse Kalrn7 | Xie et al., 2007 | N/A | AGUACAAUCCU GGCCAUGUTT |
| Sequence-based reagent | siRNA targeting sequence of mouse Kalrn9 | Yan et al., 2015 | N/A | ACUGGACUGG ACUUCUAUUTT |
| Sequence-based reagent | siRNA targeting sequence of mouse Kalrn12 | Yan et al., 2015 | N/A | CGAUGAGCUG AUGGAAGAATT |
| Sequence-based reagent | siRNA targeting sequence of mouse Strn | Breitman et al., 2008 | N/A | GGUGAAGAUCG AGAUACAATT |
| Sequence-based reagent | siRNA targeting sequence of mouse Ubr4 | Shim et al., 2008 | N/A | AAUGAUGAGC AGUCAUCUATT |
| Sequence-based reagent | qPCR primers of mouse 18s | Wang et al., 2018 | N/A | Fwd: GCTTAATTTGACT CAACACGGGARev: AGCTATCAATCTG TCAATCCTGTC |
| Sequence-based reagent | qPCR primers of mouse Gapdh | Mains et al., 2011 | N/A | Fwd: TTGTCAGCAATG CATCCTGCACCACCRev: CTGAGTGGCAGT GATGGCATGGAC |
| Sequence-based reagent | qPCR primers of mouse Ythdf2 | This paper | N/A | Fwd: GAGCAGAGA CCAAAAGGTCAAGRev: CTGTGGGCTC AAGTAAGGTTC |
| Sequence-based reagent | qPCR primers of mouse Kalrn7 | Mains et al., 2011 | N/A | Fwd: GATACCATATCCAT TGCCTCCAGGACCRev: CCAGGCTGCGC GCTAAACGTAAG |
| Sequence-based reagent | qPCR primers of mouse Kalrn9 | Mains et al., 2011 | N/A | Fwd: GCCCCTCGCC AAAGCCACAGCRev: CCAGTGAGT CCCGTGGTGGGC |
| Sequence-based reagent | qPCR primers of mouse Kalrn12 | Mains et al., 2011 | N/A | Fwd: CAGCAGCCA CGTGCCTGCAGCRev: TCTTGACATTGGG AATGGGCCGCAC |
| Sequence-based reagent | qPCR primers of mouse Strn |
This paper | N/A | Fwd: TGAAGCCTG GAATGTGGACCRev: CTATTGGGC CTCTTCACCCC |
| Sequence-based reagent | qPCR primers of mouse Ubr4 |
This paper | N/A | Fwd: TGAGTGAGG ACAAGGGCAACRev: GGGTTGGAT CGAACGAAGGT |
| Sequence-based reagent | qPCR primer for mouse Hspa12a | This paper | N/A | Fwd: GGGTTTGCACA GGCTAAGGARev: TCTGATGGACG GTCAGGTCT |
| Sequence-based reagent | qPCR primer for mouse Islr2 | This paper | N/A | Fwd: GAAGCTCCCTTA GACTGTCACCRev: CCCCATCGTGA CTCCTGCTG |
| Sequence-based reagent | PCR primer for mouse Hspa12a CDS | This paper | N/A | Fwd: ATGGCGGACAA GGAAGCTGGRev: GTAATTTAAGAA GTCGATCCCC |
| Sequence-based reagent | PCR primer for mouse Islr2 CDS |
This paper | N/A | Fwd: ATGGGGCC CTTTGGAGCRev: GCCCGCTGTC TGCCTGTAG |
| Sequence-based reagent | Mouse genotyping primers for Ythdf2 loxp site 1 | This paper | N/A | GCTTGTAGTTATG TTGTGTACCAC and GCAGCTCTGACT ATTCTAAAACCTCC |
| Sequence-based reagent | Mouse genotyping primers for Ythdf2 loxp site 2 | This paper | N/A | CTCATAACATCC ATAGCCACAGG and CCAAGAGATAG CTTTCCTAATG |
| Sequence-based reagent | Mouse genotyping primers for Six3-cre | Chunqiao Liu’s lab | N/A | CCTTCCTCCCT CTCTATGTG and GAACGAACCT GGTCGAAATC |
| Sequence-based reagent | Mouse genotyping primers for Thy1-GFP | The Jackson Laboratory website | N/A | CGGTGGTGC AGATGAACTT and ACAGACACAC ACCCAGGACA |
| Sequence-based reagent | Mouse genotyping primers for Rosa-YFP mutant site | The Jackson Laboratory website | N/A | AGGGCGAGG AGCTGTTCA and TGAAGTCGAT GCCCTTCAG |
| Sequence-based reagent | Mouse genotyping primers for Rosa-YFP wild type site | The Jackson Laboratory website | N/A | CTGGCTTCT GAGGACCG and CAGGACAAC GCCCACACA |
| Peptide, recombinant protein | Insulin | Sigma | Cat#: I6634 | |
| Peptide, recombinant protein | Recombinant Human/Murine/Rat BDNF |
PeproTech | Cat#: 450–02 | |
| Peptide, recombinant protein | Recombinant Human NT-3 | PeproTech | Cat#: 450–03 | |
| Peptide, recombinant protein | Recombinant Murine EGF | PeproTech | Cat#: 315–09 | |
| Peptide, recombinant protein | Recombinant Human FGF-basic | PeproTech | Cat#: 100-18B | |
| Commercial assay or kit | Pierce BCA Protein Assay Kit | Thermo Fisher Scientific | Cat#: 23227 | |
| Commercial assay or kit | GeneSilencer Transfection Reagent | Genlantis | Cat#: T500750 | |
| Commercial assay or kit | Magna MeRIP m6A Kit | Millipore | Cat#: 17–10499 | |
| Commercial assay or kit | EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit | Millipore | Cat#: 17–701 | |
| Chemical compound, drug | cpt-cAMP, 8-(4-Chlorophenylthio) Adenosine 3':5'-CY |
Sigma | Cat#: C3912 | |
| Chemical compound, drug |
N-acetyl-L-cysteine (NAC) |
Sigma | Cat#: A8199 | |
| Chemical compound, drug | Forskolin | Sigma | Cat#: F6886 | |
| Chemical compound, drug | Puromycin | Thermo Fisher Scientific | Cat#: A11138-03 | |
| Chemical compound, drug | Puromycin | Sigma | Cat#: P8833 | |
| Chemical compound, drug | Paraformaldehyde | Vetec | Cat#: V900894-100G | |
| Chemical compound, drug | Triton X-100 | Sigma | Cat#: V900502 | |
| Software, algorithm | GraphPad Prism 7.0 | GraphPad |
https://www.graphpad.com, RRID: SCR_002798 |
|
| Software, algorithm | STAR v2.5 | Dobin et al., 2013 |
https://github.com/alexdobin/STAR/ RRID:SCR_004463 |
|
| Software, algorithm | HTSeq | Anders et al., 2015 | https://pypi.org/project/HTSeq/ | |
| Software, algorithm | ImageJ (Fiji) | Schindelin et al., 2012 | http://fiji.sc, RRID:SCR_002285 | |
| Software, algorithm | Matlab | Matlab | https://ww2.mathworks.cn | |
| Other | TRIzol Reagent | Life | Cat#: 15596018 | |
| Other | PrimeScript RT Master Mix | Takara | Cat#: RR036B | |
| Other | 2× ChamQ Universal SYBR qPCR Master Mix | Vazyme | Cat#: Q711-02 | |
| Other | DMEM, high glucose | Gibco | Cat#: 11965–092 | |
| Other | Dulbecco’s Modified Eagle’s Medium, 10×, low glucose | Sigma | Cat#: D2429 | |
| Other | DMEM, high glucose | Hyclone | Cat#: SH30022.01 | |
| Other | Fetal Bovine Serum (FBS) | Gibco | Cat#: 10270–106 | |
| Other | Dulbecco’s Phosphate-Buffered Saline, 1× without calcium and magnesium (DPBS) | Corning | Cat#: 21–031-CVR | |
| Other | Poly-D-lysine, Cultrex | Trevigen | Cat#: 3439-100-01 | |
| Other | Laminin (mouse), Culrex | Trevigen | Cat#: 3400-010-01 | |
| Other | DMEM/F-12, GlutaMAX | Gibco | Cat#: 10565–018 | |
| Other | Neurobasal Medium, minus phenol red | Gibco | Cat#: 12348–017 | |
| Other | Penicillin-Streptomycin | Life | Cat#: 15140–122 | |
| Other | B27 serum-free supplement, 50× |
Life | Cat#: 17504044 | |
| Other | N-2 Supplement, 100× | Gibco | Cat#: 17502–048 | |
| Other | OCT Compound and Cryomolds, Tissue-Tek | SAKURA | Cat#: 4583 | |
| Other | ChemiBLOCKER | Millipore | Cat#: 2170 | |
| Other | CTB (Cholera Toxin Subunit B) conjugated by Alexa Fluor 555 |
Invitrogen | Cat#: C34776 | |
| Other | VECTASHIELD Antifade Mounting Medium with DAPI |
Vector Laboratory | Cat#: H-1200 | |
| Other | Mounting Medium, antifading (with DAPI) |
Solarbio | Cat#: S2110 | |
| Other | Normal Goat Serum | Novus | Cat#: NBP2-23475 |