Table 5. Genotyping primers.
Allele | Forward primer (5′–3′) | Reverse primer (5′–3′) |
---|---|---|
Pum2 KO | GCTGCTACTCCCTTTCTTGC | GAGCACATGTGGAGGTCAGA |
Pum2 WT and floxed | GCTGCTACTCCCTTTCTTGC | CCAAGGCGCTCAACTACTTC |
Cre | TAACATTCTCCCACCGCTAGTACG | AAACGTTGATGCCGGTGAACGTGC |
Actin | CAATAGTGATGACCTGGCCGT | AGAGGGAAATCGTGCGTGAC |
TDP43A315T | GGATGAGCTGCGGGAGTTCT | TGCCCATCATACCCCAACTG |
TDP43 | GGATGAGCTGCGGGAGTTCT | TGCCCATCATACCCCAACTG |
Control for TDP43 | CAAATGTTGCTTGTCTGGTG | GTCAGTCGAGTGCACAGTTT |