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. 2022 Feb 23;12:774988. doi: 10.3389/fonc.2022.774988

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Copyright © 2022 Shao, Piao, Wang, Guo, Wang, Wang, Tong, Yuan, Han, Fang, Zhu and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tspan9 regulates EMT, and knocking down of it impairs in vivo OS cell metastasis. (A) EMT marker protein levels and associated transcription factor expression were assessed in HOS and U2OS cells stably expressing shTspan9/OE-Tspan9 or control constructs. (B) Quantification of the Western blotting results presented in (A). (C, D) HOS cells stably expressing shTspan9 or control constructs were injected via the lateral tail vein into nude mice to establish a model of pulmonary metastasis (n=5/group). Left: representative lung images; Right: representative H&E staining results (1x and 5x). Pulmonary nodules are indicated by red arrows, while normal alveolar tissue is indicated by blue arrows. (E) Numbers of metastatic pulmonary nodules in the indicated groups. (F) Lung weights on day 28 post-HOS tumor cell injection. (G) Murine body weight was assessed every 4 days. *P < 0.05; **P < 0.01.