Table 1.
The siRNA and shRNA sequences used in this study.
| Name | Sequences | |
|---|---|---|
| Tspan9 | Forward | AACGAGAACGCCAAGAAGGA |
| Reverse | CGTTGTTCTCGGTGTGGTACA | |
| GAPDH | Forward | ATGGAAATCCCATCACCATCTT |
| Reverse | CGCCCCACTTGATTTTGG | |
| Tspan9 siRNAs | ||
| siTspan9#1 | Sense | UGGACAAGGUGAACGAGAATT |
| Antisense | UUCUCGUUCACCUUGUCCATT | |
| siTspan9#2 | Sense | CUGAAGAACGCCUGGAACATT |
| Antisense | UGUUCCAGGCGUUCUUCAGTT | |
| siTspan9#3 | Sense | CCAAGAAGGACCUGAAGGATT |
| Antisense | UCCUUCAGGUCCUUCUUGGTT | |
| Negative Control (siNC) | Sense | UUCUCCGAACGUGUCACGUTT |
| Antisense | ACGUGACACGUUCGGAGAATT | |
| Tspan9 shRNAs | ||
| shTspan9#1 | 5’-3’ | TGGACAAGGTGAACGAGAA |
| shTspan9#2 | 5’-3’ | CTGAAGAACGCCTGGAACA |
| Negative Control (shNC) | 5’-3’ | TTCTCCGAACGTGTCACGT |