TABLE 1.
Inactivation of HSV-2 infectivity by SDS
| % SDS during treatmenta | Final % SDSb | No. of plaques/platec |
|---|---|---|
| 0 | 0 | 57, 73, 343, 145, 145 |
| 1 × 10−1 | 1 × 10−3 | 0, 0, 0, 0, 0 |
| 5 × 10−2 | 5 × 10−4 | 0, 0, 0, 0, 0 |
| 2.5 × 10−2 | 2.5 × 10−4 | 0, 0, 0, 0, 0 |
| 1.25 × 10−2 | 1.25 × 10−4 | 0, 0, 0, 2, 81 |
| 2.5 × 10−3 | 2.5 × 10−5 | 28, 54, 322, 145, 104 |
a
Sterile SDS stocks (40 times the treatment concentration) were added to virus aliquots to achieve the treatment concentration. After mixing, the samples were incubated at 37°C for 10 min.
b
Following SDS treatment, virus stocks were diluted 100-fold and 1-ml aliquots were immediately adsorbed onto CV-1 cells. Plaques were counted after 20 to 24 h of infection. Each number represents an average for two plates.
c
Data are values from five experiments.