TABLE 3.
Inhibition of BPV-1 morphologic transformation of C127 cells by SDS treatment of virus
| % SDS during treatmenta | Final % SDSb | No. of foci/platec
|
||
|---|---|---|---|---|
| Expt 1 (day 12) | Expt 2
|
|||
| Day 14 | Day 17 | |||
| 0 | 0 | 266 | 255 | 153 |
| 5 | 5 × 10−3 | 0 | ND | ND |
| 5 × 10−1 | 5 × 10−4 | 0 | ND | ND |
| 5 × 10−2 | 5 × 10−5 | 0 | 0 | 0 |
| 5 × 10−3 | 5 × 10−6 | 0 | 271 | 150 |
| 2.5 × 10−3 | 2.5 × 10−6 | ND | 273 | 162 |
| 5 × 10−4 | 5 × 10−7 | ND | 229 | 151 |
a
Sterile SDS stocks (40 times the treatment concentration) were added to virus aliquots to achieve the treatment concentration.
b
Following virus treatment, treated virus stocks were further diluted 1:1,000 in order to dilute the detergent; the cells in all plates remained viable.
c
In experiment 1, virus and SDS were mixed and incubated at 37°C for 30 min; in experiment 2, virus and SDS were mixed and incubated at 37°C for 10 min. ND, not done. In control plates without BPV-1, no foci appeared.