TABLE 3.

Axolotl PCR primers and amplicons

(A) Initial UTR screen
Amplicon	WT (bp)	FORWARD primer	REVERSE primer
pax6	885	pax6 UTR for 5′-CAGTGAACCTGACATGTCTCAATAC	pax6 UTR rev 5′-TATAGCTAAGAACAAGGGACGTCTG
rax	562	rax UTR for 5′-AGACTATTTCAAAGCCTGGCATAC	rax UTR rev 5′-TGACTGCAGAGTAACCCAAAAACA
(B) Rax genomic analysis (with eyeless deletion indicated)
Amplicon	WT (bp)	FORWARD primer	REVERSE primer
CNE0	155	axorax CNE0 for 5′-AAGCTCAGGCTGTCATCCCA	axorax CNE0 rev 5′-TGACTGCACACAAGAGGCTG
CNE1	732	axorax CNE1 for 5′-AGACAAGTGAGTGTGGGTCG	axorax CNE1 rev 5′-TTGCTAATTAGTGCCGGCCA
CNE2	720	axorax CNE2 for 5′-TCCACCGTTTTACCAAGCGA	axorax CNE2 rev 5′-TTTCCAGCACTGCTCAGGAG
CNE3	283	axorax CNE3 for 5′-ATGGAGAGTGCCTTCCGACA	axorax CNE3 rev 5′-TGGCTGTAAACGTGGACTCT
3′UTR1	892	axorax 3′UTR1 for 5′-TTCCCAGTAGCTACACCCCA	axorax 3′UTR1 rev 5′-AGAGTCTGCCACCAATGCTG
3′UTR2	787	axorax 3′UTR2 for 5′-TGGTTGCACCCACTTGTTCT	axorax 3′UTR2 rev 5′-TCCACAGCATCACCACTCAC
5′UTR	705	axorax 5′ UTR for 5′-TGTGGTGTCAGGAGTGGGTA	axorax 5′UTR rev 5′-AAGATGGCCTCGATGCTGTG
ex1	467	axorax ex1 for 5′-GTAGTTCAGCCGACTGCACT	axorax ex1 rev 5′-GCACTCACCTTCGAAGCTCT
ex2 (A)	602	axorax ex2.0 for 5′-AAGGCGCTGGTTTGTGACTA	axorax ex2.0 rev 5′-GCTACTGCCAGGCTCTGAAA
ex2 (B)	613	axorax ex2.1 for 5′-ATCATTCAAACGGCAGGCAG	axorax ex2.2 rev 5′-TTCGGGCAGGTTGACCTTGA
ex2 (C)	448	axorax ex2.3 for 5′-TGCCTCTTGTGTCAGTGCAC	axorax ex2.1 rev 5′-ATGCTAAGCTATGCCGGTGT
ex2 (Dwt)	426 (E) 1354 (E′)	axorax ex2.0 for 5′-AAGGCGCTGGTTTGTGACTA	axorax WTspec rev 5′-GTACACGTCCGGGTAGTGGGA
ex2 (Dmut)	429 (e)	axorax ex2.0 for 5′-AAGGCGCTGGTTTGTGACTA	axorax MUspec rev 5′-TCGCGGCTGTACAC|GGTAGTG
ex3	784	axorax ex3 for 5′-TCAGTGTCCGCATTGCTCTT	axorax ex3 rev 5′-AATGCCTGTCCAGTTACCCG
(C) Rax cDNA analysis (with *fluorescent end-label)
Amplicon	WT (bp)	FORWARD primer	REVERSE primer
cDNA ex 1–3	497	rax cDNA ex1 for 5′-ACAGCATCGAGGCCATCTTG	rax cDNA ex3 rev *5′-TGAGTCCTGCAGCTTCATTG