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. 2022 Mar 2;603(7900):343–347. doi: 10.1038/s41586-022-04470-1

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© The Author(s) 2022, corrected publication 2022

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Extended Data Fig. 3 — a, HNH active site, showing cleaved target strand. b, L1 linker docked on PAM-distal kinked gRNA–TS duplex. Two water molecules are involved within the network of interactions that stabilize the L1 helix conformation. c, RuvC active site, showing cleaved NTS, and positioning of two Mg²⁺ ions. d, RuvC DNA cleavage mechanism. This is a typical two-metal-ion mechanism as described in²⁵ and agrees with QM/MM simulations for histidine-mediated activation²⁶, and the proposed mechanisms of Cas12j and Cas12i^41,42.