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. 2022 Mar 9;13(3):220. doi: 10.1038/s41419-022-04605-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Cross-sections of freshly isolated murine aortae were analyzed by immunofluorescence for the presence of Ly6c⁺ and Sca-1⁺ progenitor cells. Within the adventitia, a substantial part of Ly6c⁺ cells co-expressed Sca-1 distinguishing them from Ly6c⁺ monocytes that derived from the bone marrow and circulate in peripheral blood. b After ARA, cross-sections of murine aortae were analyzed by immunofluorescence for the presence of Ly6c⁺/F4/80⁺ cells. The majority of F4/80⁺ macrophages also expressed Ly6c suggesting their derivation from Ly6c⁺ progenitor cells. c Confocal image of a cross-section of a cultured aortic ring from the FlkSwitch mouse. These sections were screened for F4/80⁺ cells (red immunofluorescence) and eGFP+ cells (bone marrow-derived). Quantitative analysis of these sections (n = 6) confirmed that 40% of F4/80 macrophages originate from bone marrow-derived cells (F4/80⁺/eGFP⁺) whereas 60% are generated from bone marrow-independent progenitors (F4/80⁺/GFP^-). Scale bars: in a, b 100 µm, in c 50 µm.