Table 1.
Real-time PCR primer sequences.
| Gene | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| PPARγ (m) | ATCGAGTGCCGAGTCTGTGG | GCAAGGCACTTCTGAAACCG |
| SREBP1 (m) | GGCTATTCCGTGAACATCTCCTA | ATCCAAGGGCAGTTCTTGTG |
| C/EBPα (m) | GGAACTTGAAGCACAATCGATC | TGGTAAAGGTTCTCA |
| LXRα (m) | CAGGAGACCAGGGAGGCAAC | GCAGGGCTGTAGGCTCTGCT |
| ACC (m) | TTTTCGATGTCCTCCCAAACTTT | GCTCATAGGCGATATAAGCTCT |
| FAS (m) | AGGGGTCGACCTGGTCCTCA | GCCATGCCCAGAGGGTGGTT |
| HMGCR (m) | CAGGATGCAGCACAGAATGT | CTTTGCATGCTCCTTGAACA |
| CPT-1 (m) | CTCAGTGGGAGCGACTCTTCA | GGCCTCTGTGGTACACGACAA |
| PPARα (m) | CAGGAGAGCAGGGATTTGCA | CCTACGCTCAGCCCTCTTCAT |
| AMPK (m) | GGTGGATTCCCAAAAGTGCT | AAGCAGTGCTGGGTCACAAG |
| GAPDH (m) | ATGGAAATCCCATCACCATCTT | CGCCCCACTTGATTTTGG |
| SREBP1 (h) | ACCGACATCGAAGGTGAAGT | CCAGCATAGGGTGGGTCAAA |
| ACC (h) | CATGCGGTCTATCCGTAGGTG | GTGTGACCATGACAACGAATCT |
| FAS (h) | AAGGACCTGTCTAGGTTTGATGC | TGGCTTCATAGGTGACTTCCA |
| HMGCR (h) | GACCTTTCCAGAGCAAGCAC | TCAACAAGAGCATCGAGGGT |
| CPT-1 (h) | TCCAGTTGGCTTATCGTGGTG | TCCAGAGTCCGATTGATTTTTGC |
| PPARα (h) | TCCGACTCCGTCTTCTTGAT | GCCTAAGGAAACCGTTCTGTG |
| AMPK (h) | AGGATGCCTGAAAAGCTTGA | GACAGCCGGAGAAGCAGAAAC |
| GAPDH (h) | CTCCTCCACCTTTGACGCTG | CTCTTGTGCTCTTGCTGGGG |