Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 9;12:4136. doi: 10.1038/s41598-022-07916-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Induction of muscle hyperalgesia and NETs with intramuscular MSU injection. (a) Time-series experiment of the MNT values in the MSU- or saline-injected (contralateral) hindlimbs (n = 6 in each group). (b) Representative IHC images (200 ×) of TSM tissue on day 2, indicating citH3 (green), Gr-1 (red), and DAPI (blue). (c) Western blotting analysis of citH3 and Ly6G amounts in the TSM tissues on day 2 after intramuscular injection of MSU or saline (n = 8 in each group). The relative density is normalized based on the loading control (total histone H3: total H3 and GAPDH, respectively). Scale bar = 100 μm. (d) ELISA analysis was performed to evaluate protein levels of IL-1β, using MSU- or saline-injected TSM tissues on day 2 (n = 10 in each group). All data are shown as Tukey’s boxplots with individual data points and analyzed using paired t-test or two-way ANOVA with Tukey’s post-hoc multiple comparison test for time-series of MNT values. Statistical significance is indicated with * (p < 0.05), ** (p < 0.01), and *** (p < 0.001).