Skip to main content
. 2022 Mar 9;13:1225. doi: 10.1038/s41467-022-28844-1

Fig. 2. SIRT2 is critical for remyelination of the mice in vivo.

Fig. 2

a SIRT2 protein level in brains of SIRT2−/− and WT mice (n = 3). b–e Images and quantification of proliferating OPCs (b, c, Olig2+Ki67+, n = 3) cultured for 36 h and differentiated oligodendrocytes (d, e, Olig2+MBP+, n = 3) cultured for 48 h. Scale bars, 50 μm. f Schematic diagram of the experiment for testing OPC proliferation in vivo at 5 dpl. g–j Images and quantification of oligodendrocyte lineage cells (Olig2+) and proliferating OPCs (arrows, Ki67+Olig2+) within the demyelination lesions (dotted line) at 5 dpl (n = 5 for WT group, n = 4 for the SIRT2−/− group). Scale bar, 50 μm. k Schematic diagram of the experiment for testing OPC differentiation in vivo at 10 dpl. l–o Images and quantification of oligodendrocyte lineage cells (Olig2+) and differentiated oligodendrocytes (arrows, CC1+Olig2+) within the demyelination lesions (dotted line) at 10 dpl (n = 4 for WT group, n = 5 for the SIRT2−/− group). Scale bar, 50 μm. p Experiment design for testing remyelination efficiency in vivo at 21 dpl. q TEM micrographs of normal myelinated, demyelinated and remyelinated axons. Scale bar, 200 nm. r TEM micrographs within the lesions at 21 dpl. Scale bar, 1 μm. s–x Quantification of the proportion of remyelinated axons (s), myelin pathology level (t), G-Ratio average (u), individual G-Ratio distribution (v, linear regression) and distance between DL (w and x) within the lesions at 21 dpl (n = 6 for the WT young group, n = 5 for the SIRT2−/− group, n = 7 for the WT old group). All data are presented as mean ± SEM. The center, upper and lower line represent the median, upper and lower quartiles, respectively (x). *p < 0.05, **p < 0.01, ***p < 0.001 by two-tailed t-test (c, e, h–j, m–o) or one-way ANOVA followed by Tukey’s post hoc test (s, u, w, x) or two-way repeated ANOVA followed by Sidak’s post hoc test (t). In all instances ***p  <  0.001. n.s. no significance. In (c), **p = 0.006; in (i), *p = 0.03; in (j), *p = 0.01; in (o), **p = 0.005; in (s), **p = 0.001 (SIRT2−/− vs. WT Old); in (u), **p = 0.004 (WT Young vs. SIRT2−/−), *p = 0.02 (SIRT2−/− vs. WT Old); in (x), **p = 0.03 (WT Young vs. WT Old).