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. 2022 Mar 9;12:3811. doi: 10.1038/s41598-022-07535-3

Figure 4.

Figure 4

Endosymbiotic bacteria of the mite Fragariocoptes setiger, fluorescence in situ hybridization (FISH) with different fluorophores and oligonucleotide probes (a–h) and TEM microscopy (i–l). Mite anterodorsal (a–e) and anterolateral parts (f–h); intermuscular bacteria (d-e, yellow arrowheads), bacteria surrounding gigantic parenchymal cells (g, h, yellow arrowheads) and salivary glands (g, h, white arrowheads); DAPI + no probe (a, f); CY3 + 16S.1722F.Agr.tum (b, g); FITC + Eub338 (c, g); CY3 + 16S.1722F.Agr.tum, DAPI (d); CY3 + 16S.1722F.Agr.tum, FITC + eub338, DAPI (e). Bacterial morphotype 1 (Wolbachia) (i, j, red arrowheads), and bacterial morphotype 2 (yellow arrowheads) (k, l, yellow arrowheads) in various locations inside the mite: mid-lateral opisthosoma with saw-like cuticle and underlying tissues are visible (i), same as previous, a gigantic parenchymal cell (fat body) is shown and traced by blue arrows (l), spaces between the fat body and the gut (j, k).