FIGURE 2.

Effects of loading GV oocytes with glycine or glycine plus melatonin during vitrification, thawing and in vitro maturation on the intracellular ROS levels of the resultant oocytes. (A): Representative images of ROS staining (green) in oocytes of each experimental group. DIC, differential interference contrast image; Merge, overlapping of green and DIC. Scale bar: 200 μm. (B): The relative fluorescence intensity of ROS staining in oocytes with various treatments. All experiments were performed in triplicate. In each set of experiments, the fluorescence intensity of ROS staining was normalized to the value of oocytes in the control group. Each column presents the mean ± SEM. Different lowercase letters above columns indicate statistical difference at p < 0.05. The same lowercase letters above columns denote that the data are not significantly different at p > 0.05. Control: oocytes were neither vitrified/thawed nor supplemented with glycine or melatonin to assist their maturation; Vitrified: oocytes were sequentially vitrified, thawed and cultured for maturation without glycine or melatonin supplementation; Vitrified + Gly: oocytes were sequentially vitrified, thawed and cultured for maturation with glycine supplementation; Vitrified + Gly + MT: oocytes were sequentially vitrified, thawed and cultured for maturation with glycine plus melatonin supplementation.