FIGURE 3.

Effects of glycine or glycine plus melatonin addition during vitrification, thawing and in vitro maturation on the migration of cortical granules (CGs) in the resultant oocytes. (A): Representative images of the distribution of CGs in oocytes that were collected 44 h after maturation in each examined group. Green, cortical granules (CGs); Blue, chromatin; Merge, overlapping of green and blue. Scale bar: 40 μm. (B): The percentages of oocytes with the normal distribution of GCs in each experimental group. All experiments were performed in triplicate. Data are presented as mean ± SEM of three independent experiments. Different lowercase letters above columns indicate statistical difference at p < 0.05. The absence of letters denotes that the data are not significantly different at p > 0.05. Control: oocytes were neither vitrified/thawed nor supplemented with glycine or melatonin to assist their maturation; Vitrified: oocytes were sequentially vitrified, thawed and cultured for maturation without glycine or melatonin supplementation; Vitrified + Gly: oocytes were sequentially vitrified, thawed and cultured for maturation with glycine supplementation; Vitrified + Gly + MT: oocytes were sequentially vitrified, thawed and cultured for maturation with glycine plus melatonin supplementation.