FIGURE 5.

Effects of loading GV oocytes with glycine or glycine plus melatonin during vitrification, thawing and in vitro maturation on mitochondrial distribution and activity in the resultant oocytes. (A): Fluorescent staining of functional mitochondrial (red) counterstained with DAPI (blue) in oocytes. Merge, overlapping of red and blue. Scale bar: 20 μm. (B): Percentages of oocytes with the normal mitochondrial distribution. (C): Mitochondrial activity of oocytes with different experimental treatments. Control: oocytes were neither vitrified/thawed nor supplemented with glycine or melatonin to assist their maturation; Vitrified: oocytes were sequentially vitrified, thawed and cultured for maturation without glycine or melatonin supplementation; Vitrified + Gly: oocytes were sequentially vitrified, thawed and cultured for maturation with glycine supplementation; Vitrified + Gly + MT: oocytes were sequentially vitrified, thawed and cultured for maturation with glycine plus melatonin supplementation. All experiments were performed in triplicate. Each column presents the mean ± SEM. Different lowercase letters above columns indicate statistical difference at p < 0.05. The same lowercase letters above columns denote that the data are not significantly different at p > 0.05.