FIGURE 6.

Glycine or glycine plus melatonin supplementation during vitrification/thawing and in vitro maturation increased the relative transcript levels of glycolytic genes in the CCs. In each set of experiments, the transcript levels of the glycolytic genes of each group were normalized to the value of CCs in the control group. Control: oocytes were neither vitrified/thawed nor supplemented with glycine or melatonin to assist their maturation; Vitrified: oocytes were sequentially vitrified, thawed and cultured for maturation without glycine or melatonin supplementation; Vitrified + Gly: oocytes were sequentially vitrified, thawed and cultured for maturation with glycine supplementation; Vitrified + Gly + MT: oocytes were sequentially vitrified, thawed and cultured for maturation with glycine plus melatonin supplementation. Each column indicates the mean ± SEM. Different lowercase letters above columns indicate statistical difference at p < 0.05. The same lowercase letters above columns denote that the data are not significantly different at p > 0.05.