FIGURE 7.

Effects of loading GV oocytes with glycine or glycine plus melatonin during vitrification, thawing and in vitro maturation on the relative ATP levels in oocytes and CCs. In each set of experiments, the ATP contents of differently treated oocytes or CCs were normalized against the value of oocytes or CCs in the control group, respectively. Control: oocytes were neither vitrified/thawed nor supplemented with glycine or melatonin to assist their maturation; Vitrified: oocytes were sequentially vitrified, thawed and cultured for maturation without glycine or melatonin supplementation; Vitrified + Gly: oocytes were sequentially vitrified, thawed and cultured for maturation with glycine supplementation; Vitrified + Gly + MT: oocytes were sequentially vitrified, thawed and cultured for maturation with glycine plus melatonin supplementation. All experiments were performed in triplicate. Each column indicates the mean ± SEM. Different lowercase letters above columns indicate statistical difference at p < 0.05. The same lowercase letters above columns denote that the data are not significantly different at p > 0.05.