Figure 1.

Renal function and survival rates were assessed in male and female rats. (A) Scr levels were measured in male and female rats for 4 consecutive days after 60 min of ischemia or in male rats undergoing bilateral nephrectomy (bilat. neph). Two-way ANOVA followed by Bonferroni’s post-hoc test, ^* p < 0.05, vs. female+IRI. The dashed line shows the baseline Scr level, n = 6. (B) Survival rates of male and female rats subjected to 7 consecutive days of ischemia for 60 min. (C) Scr levels in male and female rats subjected to 4 consecutive days of ischemia for 50 min. Two-way ANOVA followed by Bonferroni’s post-hoc test, ^* p < 0.05, vs. female+IRI. (D) Survival rates of male and female rats after seven consecutive days of ischemia for 50 min. (E) Scr levels in male and female rats, OVX rats, and OVX rats that received E₂ after being subjected to 45 min of ischemia for four consecutive days. Two-way ANOVA followed by Bonferroni’s post-hoc test, ^* p < 0.05, vs. female+IRI; ^# p < 0.05, OVX+IRI vs. OVX+E₂+IRI. (F) Survival rates of male and female rats, OVX rats, and OVX+E₂ rats that were subjected to 45 min of IRI for seven consecutive days, n = 8 for each group. The data are presented as the mean ± SEM.