FIGURE 3.

Compression force enlarged the cell area of MDA-MB-231 cells on a low-adhesive surface. (A) The cell area of MDA-MB-231 cells significantly enlarged with increasing compression force from 0 mmHg (left) to 0.08, 0.16, and 0.24 mmHg (right) after culturing for 1 h. (B) An MDA-MB-231 cell showing enlarged membrane protrusions which increased with time under 0.24 mmHg compression force. (C) Quantitative analysis of the individual cell area after 1 h treatment with various compressive stress values. The cell area significantly increased from 401.67 ± 44.59 μm² (0 mmHg) to 1,300.06 ± 307.37 μm² (0.24 mmHg). Scale bar = 100 μm *p < 0.05; ***p < 0.005. Student’s t-test, two independent experiments.