TABLE 3.

Published DNA molecular markers developed for determination of cannabis chemotypes and their applicability to discriminate between different chemotypes of MX-CBD-11 and MX-CBD-707.

Marker	Reference	Primer sequence	MX-CBD-11		MX-CBD-707
			Type II	Type III	Type II	Type III
D589	Staginnus et al. (2014)	For CCTGAATTCGACAATACAAAATCTTAGATTCAT	Yes	No	Yes	Yes*
		Rev ACTGAATATAGTAGACTTTGATGGGACAGCAACC	Yes	No	Yes	Yes*
B1080/B1192	Pacifico et al. (2006)	For AAGAAAGTTGGCTTGCAG	Yes	No	Yes	Yes*
		THCAS-specific-Rev TTAGGACTCGCATGATTAGTTTTTC	Yes	No	Yes	Yes*
B190/B200	de Meijer et al. (2003)	For TGCTCTGCCCAAAGTATCAA	No*	No*	No*	No*
		Rev CCACTCACCACTCCACCTTT	No*	No*	No*	No*
THCA583-For	Weiblen et al. (2015)	For GTG GAG GAG GCT ATG GAG C	Yes	Yes*	Yes	Yes*
THCA1034-Rev		Rev CCC AAC TCA GGA AAG CTC TTG	Yes	Yes*	Yes	No

Asterisks (*) mark discrepancies in the expected versus obtained results, because primers D589, B1080/B1192, and THCA583-For/THCA1034-Rev should amplify parts of the functional THCAS gene, while marker B190/B200 should amplify parts of the THCAS (190 bp) and CBDAS (200 bp) genes.