Figure 2.

(A) SOD1-G93A transgenic mice and control wild-type (wt) C57Bl/6 mice were inoculated i.c. with 5 x 10⁸ plaque forming unit viral particles of ATCV-1 (n = 9; 50 μl) or PBS (n = 9; 50 μl) at 35 days of age. (B) A second cohort of SOD1-G93A transgenic mice were inoculated i.c with PBS (n = 5) or 5 x 10⁸ heat-killed (HK) ATCV-1 (n = 5). (C) A third cohort of SOD1G93A transgenic mice were inoculated i.c with PBS (n = 10) or poly I:C(20 ug) (n = 20). (Left graphs) MND evaluation: level 1: Collapse/partial collapse of hind leg toward midline; level 2: Toe curl during walking or foot-dragging; level 3: Rigid hind limb paralysis or minimal joint movement for forward motion; level 4: Failure to right within 30 s. (data analyzed by Student's t-test; * indicates significance p ≤ 0.05), (Right graphs) Kaplan-Meier Survival data of transgenic inoculated mice.