Figure 2.

CX3C chemokine receptor 1–enhanced green fluorescence protein positive (CX3CR1-EGFP⁺) cell morphologic tissue polarization lags in the colon as compared with that in the small intestine. Morphologic polarization of grafted CX3CR1-EGFP⁺ cells in the crypt/mucosa and the serosa/muscularis layers of the small intestine and the colon was analyzed. A: Representative confocal microscopy images depict the sequential changes in the polarization state of grafted CX3CR1-EGFP⁺ cells. B–D: Quantitative analyses of the small intestinal (B and C) and the colon crypt/mucosa and the serosa/muscularis layer (D and E) depict a gradual shift from mostly ameboid to ramified morphology of grafted cells in the tissue over the 4 weeks after BMT. Scale bar = 20 μm.