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. 2022 Feb;192(2):295–307. doi: 10.1016/j.ajpath.2021.10.013

Figure 6.

Figure 6

Expression analyses confirm increased macrophage colonization of the colon and the brain by dextran sodium sulfate (DSS)-induced colon injury. Transcription analyses by quantitative RT-PCR for common macrophage markers were performed in steady-state (control) and DSS-inflamed colon and the brain. A and B: Quantitative analyses of CX3C chemokine receptor 1 (CX3CR1), CD64, and C-C chemokine receptor type 2 (CCR2) in the colon (A) and the brain (B) showed significant increases in transcript levels after DSS treatment, consistent with an increased number of engrafting CX3CR1-enhanced green fluorescence protein positive (EGFP)+ cells in both colon and brain tissue. C: Transcription analyses of more selective brain macrophage and microglia markers ionized calcium binding adaptor molecule 1 (Iba1) and transmembrane protein 119 (TMEM119) showed similar increases after DSS treatment in the acute (week 2) but not resolution (week 4) phase. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. BMT, bone marrow transplantation; Wk, week.