Supplemental Figure S1.

Levels of transforming growth factor (TGF)-β1 measured by enzyme-linked immunosorbent assay in two different culture conditions. A: Total TGF-β1 in culture medium in a matrix with and without collagen XII shows increased total levels of TGF-β1 in the absence of collagen XII. B: Total TGF-β1 in culture medium in a matrix where collagen XII synthesis is blocked after a matrix containing collagen XII is formed to simulate a rescue experiment shows no significant difference in TGF-β1 expression. We ascribe the differences in TGF-β levels to different culture time and cell manipulation needed to induce Col12a1 silencing. n = 3 (A). ∗∗∗∗P < 0.0001. WT, wild type.