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shRNA silencing of collagen XII expression in stromal fibroblast primary cultures. A: Real-time PCR of fibroblast cell culture transfected with three different constructs shows significant silencing of Col12a1 expression. B: Protein blots confirm significant silencing of Col12a1 expression after puromycin selection in primary cultures. C: Cells in primary cultures 48 hours after lentivirus transduction. Scale bar = 40 μm (C). Ctrl, control.
