Skip to main content
. 2022 Feb 20;24:729–741. doi: 10.1016/j.omto.2022.02.012

Figure 1.

Figure 1

Functional characterization of the novel IL13 CAR in Jurkat T cells

(A) Illustration of a cell-based luciferase reporter system for analyzing the biological activity of IL13 CAR. When a CAR expressed on the surface of Jurkat T cells (named the effector cell) carrying a luciferase reporter driven by an NFAT-response element was activated by its partner molecule present on the target cell, the luciferase expression level increased. (B) The biological activity assay of different CARs with U87 or THP-1 target cells using a cell-based luciferase reporter system. (C) The biological activity assay of different CARs with IL13Rα2-or IL13Rα1-engineered K562 target cells using a cell-based luciferase reporter system. Shown are representative plots of three independent experiments performed in triplicate. Statistically significant differences are indicated: ∗p < 0.05; ∗∗p < 0.01. IL13 (2MS) stands for IL13 (E13K.R109K), while IL13 (4MS) stands for IL13 (E13K.R66D.S69D.R109K).