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. 2022 Feb 20;24:729–741. doi: 10.1016/j.omto.2022.02.012

Figure 3.

Figure 3

Functional characterization of the novel bispecific IL13 (4MS)-EphA2 scFv-TanCAR in human primary CD8+ T cells

(A) The detection of the CAR expression level on the surface of human primary CD8+ T cells by flow cytometric assay with anti-Flag antibody. (B) The detection of IL2 levels in the supernatants from different groups of CD8+ CAR-T cells co-cultured with U87 cells or THP-1 cells. (C) The detection of IFNγ levels in the supernatants from different groups of CD8+ CAR-T cells co-cultured with U87 cells or THP-1 cells. (D) The detection of IL2 levels in the supernatants from different groups of CD8+ CAR-T cells co-cultured with U87 cells. (E) The detection of IFNγ levels in the supernatants from different groups of CD8+ CAR-T cells co-cultured with U87 cells. (F) The measurement of the cytotoxic activity of different groups of CD8+ CAR-T cells for U87 cells by LDH level assay. (G and H) The measurement of the cytotoxic activity of different groups of CD8+ CAR-T cells for THP-1 cells by LDH level assay (H) The measurement of the cytotoxic activity of different groups of CD8+ CAR-T cells for U87 cells by LDH level assay. Shown are representative plots of three experiments performed in triplicate. Statistically significant differences are indicated: ∗p < 0.05; ∗∗p < 0.01. TanCAR stands for IL13 (4MS)-EphA2 scFv-TanCAR.