Figure 6.

Silencing SENP1 upregulates the expression of A20 and ferroptosis-related genes in A549 cells. Transcriptomic profile of SENP1-silenced A549 cells compared to control cells (A). (B) Western blot was used to detect the ferroptosis-related proteins ACSL4, GPX4, A20 and P53 in SENP1-silenced A549 cells or control cells treated with 5 µM erastin for 24 h. (C) Western blot was used to detect the ferroptosis-related proteins ACSL4, GPX4, A20, and P53 in SENP1-silenced A549 cells treated with cisplatin for 24 h. (D) Immunohistochemical staining assay (×100): the tissues were blocked and incubated with the primary antibody, second antibody and several staining steps. the expression of ACSL4, GPX4, A20, and SLC7A11 in tumor tissues of nude mice grafted with SENP1 overexpressed A549 cells treated with cisplatin.