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. 2022 Feb;10(4):224. doi: 10.21037/atm-21-6909

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2022 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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A20 SUMOylation and its effect on growth of lung cancer cells. (A) A20 and SUMO-1 in SENP1-silenced A549 cells were detected by western blotting. (B) A20 and SUMO-1 in A20-overexpressed A549 cells were detected by western blotting. (C) A20 immunoprecipitation of A549 cells was detected for SUMO1 by western blotting. (D) The proliferation of A549 cells transduced with A20 or a control vector was detected by CCK8 assay. (E) The ROS generation of A549 cells transduced with A20 or a control vector treated with erastin and 0.5 µm Fer-1. (F) The cell viability of A549 cells transduced with A20 or a control vector treated with erastin was detected by CCK8 assay. *, P<0.05 vs. control vector; **, P<0.01 vs. control vector.