Figure 2.

Effect of phycocyanin on the expression of c-myc and CyclinD1 in Hp-infected AGS cells. (A) The relative expression of c-myc and CyclinD1 transcripts in AGS cells 8 h after Hp infection was determined by qPCR. The mRNA levels were normalized to GAPDH levels. (B) Western blot analysis for c-myc and CyclinD1 expression in AGS cells after Hp infection. GAPDH was used as a loading control (**, P<0.01 vs. NC group; ^#, P<0.05; ^##, P<0.01 vs. Hp group). NC, AGS cells were neither infected with Hp nor treated with phycocyanin; PC, cells were treated with only 150 µM phycocyanin; Hp, cells were infected with Hp at ratio of 50:1 (cells/bacteria) for 12 h; PC + Hp, Hp infection of AGS cells after pre-treatment with 150 µM phycocyanin for 24 h. Experiments were performed in triplicate for each data set. GAPDH was used as an internal control.