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. 2022 Feb 24;13:857869. doi: 10.3389/fphar.2022.857869

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Copyright © 2022 Zou, Chen, Li, Li, Wei, Li, Wang, Chen and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EcN-MT reduced liver inflammation and apoptosis. Values are presented as means ± SD (n = 3, except q-PCR results repeated five times). (A) Western bloting analysis of TLR4, MyD88, p-p65, p65 expression in liver tissues. The relative expressions of (B) TLR4, (C) MyD88, (D) p-p65/p65 were quantified by ImageJ. β-actin was used as an internal control. (E) Western bloting analysis of Bax, Bcl-2 expression in liver tissues. (F) The relative expressions of Bcl-2/Bax were quantified by ImageJ. β-actin was used as an internal control. The relative mRNA expressions of (G) IL-1β, (H) IL-6, (I) TNF-α in liver tissues were detected by q-PCR. *p < 0.05, **p < 0.01, ***p < 0.001.