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Scheme of short breeding cycle using CRISPR/Cas9 in rice. Agrobacterium-mediated transformation used for genome editing with CRISPR/Cas9. In regenerated plants, a gene was selected and edited by T-DNA insertion and sequencing. On the T0 plant, each spike is assigned a different accession number. T1 plants are transplanted to one row for each line. T2 seeds are harvested in bulk.
