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. 2022 Feb 24;13:829566. doi: 10.3389/fpls.2022.829566

FIGURE 5.

FIGURE 5

Saccharomyces cerevisiae Y2H analysis of protein-protein interactions between CaNINJA and Rupali or Genesis836 alleles of CaTIFY4B. (A) Control plate. (B) Test for URA3 reporter gene activation. (C) Test for HIS3 reporter gene activation. (D) Test for lacZ reporter gene activation. Plates in panels (B–D) were replica plated from plate (A); growth on these plates indicates a positive protein-protein interaction. Each patch is derived from a single transformed colony; patches for 1, 4, and 6 are negative controls while 5 (GAL4-Krev1-RalGD) is a strong positive control. Details for each of the construct interactions are provided in Supplementary Table 1.